Title An Escherichia coli expression system for glutamyl endopeptidasesoptimized by complete suppression of autodegradation
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چکیده
Title An Escherichia coli expression system for glutamyl endopeptidases optimized by complete suppression of autodegradation Author(s) Ono, Toshio; Nemoto, Takayuki K; Shimoyama, Yu; Kimura, Shigenobu; Ohara-Nemoto, Yuko Citation Analytical Biochemistry, 381(1), pp.74-80; 2008 Issue Date 2008-10-01 URL http://hdl.handle.net/10069/19225 Right Copyright (c) 2008 Elsevier Inc. All rights reserved. NAOSITE: Nagasaki University's Academic Output SITE
منابع مشابه
An Escherichia coli expression system for glutamyl endopeptidases optimized by complete suppression of autodegradation.
V8 protease (GluV8), a member of the glutamyl endopeptidase I family isolated from the V8 strain of Staphylococcus aureus, is widely used for proteome analysis because of its unique substrate specificity and resistance to detergents. We recently developed an Escherichia coli expression system for the production of GluV8 based on a technique that suppresses the autoproteolysis--the use of the pr...
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V8 protease, a member of the glutamyl endopeptidase I family, of Staphylococcus aureus V8 strain (GluV8) is widely used for proteome analysis because of its unique substrate specificity and resistance to detergents. In this study, an Escherichia coli expression system for GluV8, as well as its homologue from Staphylococcus epidermidis (GluSE), was developed, and the roles of the prosegments and...
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